Molecular Biology 3 - Plasmids
MolBio 3 Companion Questions
Learning Objectives and Terms
- Genome - all the hereditary information
- Chromosome - 1 piece of genomic DNA
- Know the 2 requirements of a chromosome for maintenance
- Know the function of ORIs
- Know that the 2 required elements of a plasmid are an ORI and an antibiotic resistance gene.
- Know the difference between and antibiotic and and antibiotic resistance gene.
- Know that ORIs can be host specific
- Know what Restriction Enzymes are
- Know what Restriction SITEs are
- Know what a MCS (multiple cloning site) is
- Know what Blunt and sticky ends are
- Given a table of restriction sites, be able to determine if the resulting cut is blunt or sticky
Additional Terms